AmplifyRP  Sharka-PPV

Plum pox virus (Sharka disease) is an aphid transmitted potyvirus that can cause significant loss of yield and marketability of stone fruit (peaches, plum, cherry, etc). PPV is found around the world and is of particular importance in Europe and other Mediterranean regions. PPV detections have been confirmed in the U.S. and Canada in the late 1990’s and early 2000’s which instigated large scale testing and eradication programs due to the severity of the disease.

About The Assay:  AmplifyRP® Acceler8 for PPV enables users to rapidly amplify target RNA at a single operating

Plant pathogens detection by RNA / DNA analysis in 30 minutes

temperature, using only a crude sample extract, in an easy-to-use portable heat block. Amplified product is then tested for in an Amplicon Detection Chamber where clear “yes / no” results are read visually on a lateral flow strip (much like a pregnancy test). The entire testing process can be completed in as little as 30 minutes by users of any skill level.

AmplifyRP® Acceler8 for PPV was designed to run in GEB4 extraction buffer; the same extraction buffer used in Agdia’s PPV ELISA (USDA approved screening method for PPV) as well as the Flashkit® (ImmunoStrip®).  This allows molecular confirmation of ELISA or Flashkit® (ImmunoStrip®) results from the exact same plant extract.

AmplifyRP Acceler8 for PPV detects ALL known strains of the virus including PPV-C, PPV-M, PPV-D, PPV-EA, PPV-Rec, PPV-W, PPV-T, PPV-CR, PPV-An.

Reference: Rapid diagnostic detection of plum pox virus in Prunus plants by isothermal AmplifyRP® using reverse transcription-recombinase polymerase amplification. 2014, Zhang S, Ravelonandro M, Russell P, McOwen N, Briard P, Bohannon S, Vrient A.