A question? Need help?
Do not hesitate to contact us
We are pleased to announce our participation to Fruit Logistica 2015. We will be at Vegepolys (French Plant Cluster) ir booth n°E-08 in Hall 22. We are waiting for you on Friday 6th February to present you our innovations for plant disease detection.
For more information on Fruit Logistica: http://www.fruitlogistica.de/en/
We are pleased to announce the immediate availability of a new Flashkit® (ImmunoStrip®) for detection of Potato virus X (PVX) in potato.
The PVX Flashkit (code: ISK10000) is an easy-to-use detection kit that can be performed anywhere, including the field, to test suspect plants for the pathogen. It is specific to PVX and is not known to cross react with other pathogens in potato.
Conveniently, The PVX Flashkit may also be used simultaneously with the PVY Flashkit in the same sample extract. This is specially useful for seed certification inspectors or growers who want to quickly identify either virus in a plant. Dual PVX and PVY infections are known to cause more severe disease symptoms and can cause increased yield loss.
New !! Flashkit ® Infographics
Agdia-Emea Flashkits do not require laboratory experience or equipment to run. The entire testing process including sample preparation takes only 15-30 minutes. This helps facilitate faster and more informed management decisions by end-users. Feel free to contact us for more information on the PVX Flashkit and other potato tests: info@agdia-emea.com
We announce
the commercialization of a new rapid lateral flow test strip which was developed to detect Vip3A protein expressed in transgenic corn, event MIR162 developed by Syngenta Seeds, Inc. (SYN-IR162-4). This protein confers resistance to feeding damage caused by lepidopteran insects by selectively damaging their midgut.
The Vip3A ImmunoStrip® was specifically developed and validated for trait confirmation testing of seed lots using either individual seeds or leaf material. This ImmunoStrip® may also be used for testing bulk grain samples with a detection limit of 1 Vip3A kernel in 500 negative kernels (0.2%). All Agdia ImmunoStrips are easy to use and are rugged enough for field environments and results are available in as little as 5 minutes.
The Vip3A ImmunoStrip® (code: STX83500) is ready for immediate sale and complements Agdia-Emea’s comprehensive portfolio of validated, easy-to-use ImmunoStrips for identifying transgenic traits.
For more information about this test, please contact us:
Email to: info@agdia-emea.com
Phone: ++ 33 (0) 1 60 78 81 64
AmplifyRP Sharka-PPV
Plum pox virus (Sharka disease) is an aphid transmitted potyvirus that can cause significant loss of yield and marketability of stone fruit (peaches, plum, cherry, etc). PPV is found around the world and is of particular importance in Europe and other Mediterranean regions. PPV detections have been confirmed in the U.S. and Canada in the late 1990’s and early 2000’s which instigated large scale testing and eradication programs due to the severity of the disease.
About The Assay: AmplifyRP® Acceler8 for PPV enables users to rapidly amplify target RNA at a single operating
Plant pathogens detection by RNA / DNA analysis in 30 minutes
temperature, using only a crude sample extract, in an easy-to-use portable heat block. Amplified product is then tested for in an Amplicon Detection Chamber where clear “yes / no” results are read visually on a lateral flow strip (much like a pregnancy test). The entire testing process can be completed in as little as 30 minutes by users of any skill level.
AmplifyRP® Acceler8 for PPV was designed to run in GEB4 extraction buffer; the same extraction buffer used in Agdia’s PPV ELISA (USDA approved screening method for PPV) as well as the Flashkit® (ImmunoStrip®). This allows molecular confirmation of ELISA or Flashkit® (ImmunoStrip®) results from the exact same plant extract.
AmplifyRP Acceler8 for PPV detects ALL known strains of the virus including PPV-C, PPV-M, PPV-D, PPV-EA, PPV-Rec, PPV-W, PPV-T, PPV-CR, PPV-An.
Reference: Rapid diagnostic detection of plum pox virus in Prunus plants by isothermal AmplifyRP® using reverse transcription-recombinase polymerase amplification. 2014, Zhang S, Ravelonandro M, Russell P, McOwen N, Briard P, Bohannon S, Vrient A.
Job Title: Account Manager Vegetable, Citrus, and Seed Health
Department/Group: Sales and Marketing
Location: Elkhart, Indiana, USA.
Travel Required: Yes
Position Type: Full-time
Job Description
Agdia, Inc is the leading developer and manufacturer of test kits for detection of plant pathogens and transgenic traits (GMO’s). Agdia products and services are used all over the world by an extensive range of clientele who are responsible for assessing crop quality. Agdia’s mission is to assist all facets of crop production grow high quality, healthy, and profitable crops through routine testing and management of plant disease and transgenic traits.
Job Purpose:
Agdia is seeking a motivated and results driven individual to strengthen and expand our market share in domestic and international vegetable, citrus, and seed health markets. Agdia serves more than 135 countries with diagnostic products and services through direct sales as well as a network of distributors. The person filling this position will ultimately be responsible for identifying new business opportunities and growing annual revenues within their designated area(s) of responsibility (market segments).
Duties:
– Maintain a working relationship with an already established customer base in person and via phone and emails
– Cold calling to arrange teleconferences or face-to-face meetings with new prospects
– Understanding clients’ business operations as it relates to plant health and diagnostic needs.
– Representing Agdia at tradeshows or technical meetings
– Gathering market and customer intelligence and disseminating that information within a CRM system and via other communication channels where necessary
– Advise in new product development and / or product improvements based on customer / market intelligence
– Negotiating price and delivery with customers
– Prepare quotations and / or taking orders from customers
– Travel domestically and internationally where applicable.
– Routinely review accounts for sales performance and convey buying trends to employers
– Attending team project and / or sales meetings
– Update job knowledge by participating in educational opportunities; reading trade publications.
– Work in a capacity where he or she takes ownership for their responsibilities, successes, and failures.
– Grow Agdia’s market share and revenues with existing and new client base
Skills/Qualifications:
– Bachelor’s degree in a Plant Pathology, business, or marketing related field.
– Experience in horticulture and / or plant pathology
– Sales and / or marketing experience in addition to horticulture and / or plant pathology would be a huge plus
– Must be proficient in the use of Microsoft office suite.
– Ability to speak Spanish would be a plus.
– Must have an acute attention to detail
– Must be outgoing, willing to share ideas and contribute to a team working environment.
Applications accepted by: Fax or Email to Mr Keith Schuetz, Marketing Director.
Fax: +1 574-264-2153 / kschuetz@agdia.com
www.agdia.com
Maize lethal necrosis (MLN) is a disease of maize caused by the combination of Maize chlorotic mottle virus (MCMV) and a virus belonging to the potyviridae family. The most commons are the potyvirus Sugarcane Mosaic Virus (SCMV), Maize Dwarf Mosaic Virus (
) or the tritimovirus Wheat Streak Mosaic Virus (WSMV).
Diffusion: The disease devastates corn fields in east Africa. It has been first described in Kenya in 2012. It has now spread to Uganda, Rwanda and Tanzania and South Sudan. The viruses are vectored by insects as maize thrips, rootworms and leaf beetles.
Symptoms: Premature plant death / Sterile male plants / Rotting cobs
Impact: As corn is the main staple food in East Africa, MLND is considered as a serious threat for food security. Moreover, corn plants infected with MLND can develop secondary fungal infections and are not considered safe to eat.
Management: Diseased plants should be immediately uprooted and burnt, to prevent further spread. Fields should be constantly monitored. Seeds should be tested before planting. Finally, resistant or tolerant varieties should be used.
Agdia-Emea diagnostic solutions for MLND
ELISA test available: We can also supply your laboratory with the ELISA tests to detect the viruses. If you don’t have a lab, we can provide you training and help you establishing an internal ELISA lab. Finally, we can recommend local laboratories in your country that use our tests to detect the MLND responsible viruses.
Flashkit® MDMV: If you want to test plants in the field, we have a rapid test called Flashkit® (ImmunoStrip®) that allows the detection of MDMV in the field. The test requires no training and it is very simple to use. The answer is provided by the test is 30 minutes or less. We are developing a similar test that will be able to detect the most of the viruses belonging to the Potyvirus group.
Testing service offer: Agdia Testing Services can tests your corn plants and seeds for the viruses that may be the cause of MLND. We can detect the 4 viruses by ELISA and the Potyviridae family by PCR.
For more pathogen tests on Corn contact us: info@agdia-emea.com
We share here with you some pictures taken during the GreenTech show in Amsterdam (10-12 June 2014) where we had a booth to present our recent innovations of Flashkits, ELISA tests and AmplifyRP. We had the pleasure to meet there some of our customers and interested contacts coming from all over the world. We hope to see you soon during the next event. We will keep you informed!
Agdia-Emea Team.
New – AmplifyRP Acceler8 for the detection of the Little cherry virus 2
Acceler8 for LChV2 is a rapid RNA amplification and detection platform designed for field-based or laboratory testing of cherry crops for Little cherry virus 2.
Little cherry virus 2 (LChV2) is the causal agent of little cherry disease in sweet cherry and is found in North America and other parts of the world (Mekuria et al, 2014). The pathogen is a mealybug-transmittedvirus and does not induce significant foliar symptoms in most sweet cherry cultivars, but does cause virus-infected trees to yield unevenly ripened small fruits with poor flavor (Mekuria et al, 2014).
AmplifyRPAcceler8 enables users to rapidly amplify and test for LChV2 RNA at a single operating temperature using only a crude sample extract. Amplified product is then tested in an Amplicon Detection Chamber where clear “yes / no” results are read visually on a lateral flow strip (much like a pregnancy test). The entire testing process can be completed in as little as 30 minutes by users of any skill level.
Notably, this test has demonstrated equivalent or, in some instances, better sensitivity than a currently used RT-PCR method (Mekuria et al, 2014) when testing samples from symptomatic trees.
AmplifyRP Acceler8 for LChV2 has been validated against multiple isolates collected throughout Washington State growing regions. It does not cross-react with other distinct pathogens known to cause symptoms of little cherry disease such as Little cherry virus 1 and Western X Phytoplasma.
Citation: T.A. Mekuria et al. / Journal of Virological Methods 205 (2014) 24-30
ASBVd is found in avocado growing regions worldwide. Trees infected with ASBVd can result in a loss of yield and / or production of unmarketable fruit. However, symptoms are not always present making pro-active testing a critical disease control measure.
Agdia’s ASBVd Nucleic Acid Hybridization Assay is a convenient and economical method for testing hundreds of trees at once. Its high sensitivity allows combining up to 8 leaves from each of 4 trees, or a total of 32 leaves, for analysis. The assay’s limit of detection is approximately 12.5 picograms of in vitro expressed ASBVd RNA.
End-users are only required to prepare the sample extracts and spot them onto a specially treated membrane, which is provided in the kit. Once this is completed, the membrane is then shipped to Agdia’s Testing Services Department where the chemiluminescent hybridization is performed by highly trained technicians. This analysis is included the price. Results are usually reported within two to four business days of our laboratory receiving the membrane for analysis.
Also Available for Avocado
For customers that wish to mail samples to Agdia Testing Services, we now offer an avocado screen that includes testing for:
Avocado sunblotch viroid (ASBVd), Phytophthora (Phyt), Xanthomonas campestris (Xc)
For more information on how to send in samples, please contact us info@agdia-emea.com.
The PPV Fla
shkit® is an on-site test for rapid detection of Plum pox virus in leaves of stone fruit species.
Plum pox virus (Sharka disease) is an aphid transmitted potyvirus that can cause significant loss of yield and marketability of stone fruit (peaches, plum, cherry, etc). PPV is found around the world and is of particular importance in Europe and other Mediterranean regions. PPV detections have been confirmed in the U.S. and Canada in the late 1990’s and early 2000’s which instigated large scale testing and eradication programs due to the severity of the disease.
Our PPV Flashkit® is easy to use and can be completed on-site with little or no skill required. Results are available in just minutes with a maximum run time of 20 minutes. A convenient property of the PPV Flashkit® is that it was designed to run in GEB4 extraction buffer; the same extraction buffer used in Agdia’s PPV ELISA (USDA approved testing method for PPV) as well as the forthcoming AmplifyRP® DNA / RNA detection platform. This allows current ELISA users to confirm results by another testing method using the same sample extract.
The PPV Flashkit® has been validated against all known strains of PPV including PPV-C, PPV-D, PPV-EA, PPV-M, PPV-rec, PPV-W, PPV-T, and PPV-CR.
Welcome on our new website!
All Agdia-Emea team hope that you will be satisfied with this new structure, we worked to offer a better product research.
Still at your disposal for information so don’t hesitate to contact us!
Agdia, Inc (Elkhart, IN), announced an offer of diagnostic testing for Soybean Vein Necrosis Virus (SVNV). SVNV is a Tospovirus that has been identified in several soybean growing states within the U.S. According to multiple online sources, it is not yet known if the pathogen has a significant impact on crop yields. Despite the uncertainties of its economic impact, stakeholders are keeping a close eye on the pathogen to gain a better understanding of what the future may bring.
Agdia has developed an Enzyme-linked immunosorbent assay (ELISA) that detects the virus with a high degree of sensitivity and specificity. Until this year, Agdia had performed SVNV testing using a Tospovirus group PCR test which required subsequent sequencing analysis to confirm if positive samples were in fact SVNV. While this testing method was effective, it also came at a high cost and often took more than a week to obtain results.
Debi Groth-Helms, Agdia’s testing Laboratory Director says, “SVNV is a very hot topic right now. Last summer we had a substantial interest in testing for this pathogen by our customers who needed an affordable method of testing. We are very pleased that we were able to meet that demand this season with a test that will enable us to provide faster turnaround times for our customers at an affordable price point. This test will play a key role in survey work that is being conducted throughout soybean growing regions”.
Agdia’s Testing Services laboratory will begin accepting soybean samples for SVNV analysis immediately. ELISA results are usually available in two business days. Agdia also plans to release a SVNV ELISA test kit at a later date this summer.
Release of the Maize Dwarf Mosaic Virus Flashkit®, an on-site tool to quickly detect the presence of MDMV in corn and other gramineous crops.
Maize dwarf mosaic virus is a potyvirus that naturally infects members of the Poaceae (grass) family, such as corn, sorghum and Johnson grass. It is transmitted by corn seed and by a broad range of aphids in a non-persistent manner. Visual symptoms include mosaic or mottle, commonly near the base of young leaves, which under hot weather may turn into chlorosis. Severe infection may induce stunting and poor seed.
The Flashkit® test is an ideal screening tool for field inspection, scouting and disease diagnosing in general. The test requires no equipment or expertise to run. Results are obtained in as little as a few minutes making them perfect for use in the field or greenhouse.
MDMV Flashkit® is specific to MDMV and does not cross-react with other pathogens that may infect gramineous, such as Barley stripe mosaic virus (BSMV), Barley yellow dwarf virus-PAV (BYDV-PAV), Cereal yellow dwarf virus-RPV (CYDV-RPV), Barley yellow dwarf virus-RMV (BYDV-RMV), Barley yellow dwarf virus-SGV (BYDV-SGV), Brome mosaic virus (BMV), Corn stunt spiroplasma (Css), Cucumber mosaic virus (CMV), High plains virus (HPV), Johnson grass mosaic virus (JgMV), Maize chlorotic mottle virus (MCMV), Maize mosaic virus (MMV), Maize stripe virus (MSpV), Maize white line mosaic virus (MWLMV), Pantoea stewartii (Pstew), Sugarcane mosaic virus (SCMV), Sugarcane mosaic virus (SCMV), Wheat spindle streak mosaic virus (WSSMV), Wheat streak mosaic virus (WSMV), Ustilago maydis (corn smut).
No host reactions were observed when testing healthy corn, Johnson grass or sorghum leaf tissue, and sorghum seeds.
Note: ImmunoStrips® is a registered trademark of Agdia Inc.. ImmunoStrips are trademarked and marketed in EMEA under the name Flashkit®.
Agdia, Inc (Elkhart, IN), has commercialized the first rapid and field deployable DNA test for Fusarium oxysporum f.sp vasinfectum, Race 4 (FOV4). Based on AmplifyRP® Acceler8TM technology, this fast, simple and easy-to-use test is specifically designed for FOV4 detection in plant stem and leaf tissue. Results are evident in as little as 30 minutes from start to finish with little skill required for lab or field testing.
AmplifyRP® Acceler8TM technology uses DNA recombinase and polymerase, along with other proteins and enzymes, to rapidly amplify target DNA or RNA at a single temperature (39°C) using only a crude sample extract, yet achieves similar sensitivity and specificity as PCR methods. In contrast to PCR, the technology does not require thermal cycling or DNA purification steps which are expensive and time consuming.
The assay is performed using only a crude extract which is mixed with amplification reagents that are prepared in a ready-to-use reaction tube. Amplification takes place by placing the reaction tube in a portable heat block for a brief period of time (15 minutes). Thereafter the entire tube is placed into a device that detects amplified target DNA template using a lateral flow strip. A positive result is indicated by having two visible lines on the lateral flow strip, one a control line and the other a test line.
The Agdia AmplifyRP Acceler8 FOV4 test resulted from a collaborative effort between the California Cotton Alliance (CCA) and Agdia, Inc. A portion of the proceeds from the sale of this test will be returned to the CCA for ongoing and future efforts to support the cotton industry.
About Agdia
A leading provider of diagnostic solutions for agriculture, Agdia, Inc. has been serving plant breeders, propagators, growers, universities, and private testing laboratories since 1981. The company offers a comprehensive portfolio of validated, easy-to-use diagnostics for identifying plant pathogens, hormones, and transgenic traits, as well as in-house testing services.
About California Cotton Alliance
California Cotton Alliance is a nonprofit foundation that provides funding for cotton research, agricultural education, scholarships and grants. Our goal is to provide support for the cotton growers, its related industry and all agriculture within California.
