Detection of Plum Pox Virus / Sharka Virus / PPV

Plum pox virus (PPV) responsible of Sharka disease is a potyvirus. Main host plants are stone fruits like apricot trees, plum trees, peach trees and sometimes almond trees. PPV can cause significant loss of yield and marketability of those stone fruits. This quarantine virus infects also the major part of ornamental prunus like cherry plum (myrobalan plum), blackthorn, damson plum, Nankin cherry…

PPV is found around the world especially in Europe and Mediterranean regions.

Sharka virus is transmitted through aphids or grafting. Symptoms can appear on leaves, fruits or flowers: spots, stripes or chlorotic rings, vein clearing and leaf deformations in peach trees.

Available kit for the detection of PPV:

Flashkit®

The PPV Flashkit^® has been validated against all known strains of PPV including PPV-C, PPV-D, PPV-EA, PPV-M, PPV-rec, PPV-W, PPV-T, and PPV-CR.

Reagent set

This test can be used to detect the Plum pox virus (PPV) in symptomatic or asymptomatic leaves, fruit, and flowers of stone fruits. Our PPV test detects the following PPV isolates: PPV-C (cherry isolate) PPV-M, PPV-D, PPV-EA, PPV-Rec, and PPV-W.

Only SRA tests for Double Antibody Sandwich (DAS) ELISA

Capture and detection antibodies :Polyclonal mixture

Positive and negative controls are available.

AmplifyRP®

AmplifyRP^® Acceler8^® for PPV is a rapid RNA amplification and detection platform designed for field-based or laboratory testing of prunus crops for Plum pox virus. AmplifyRP^® Acceler8^® enables users to rapidly amplify and test for PPV RNA at a single operating temperature using only a crude sample extract. AmplifyRP^® Acceler8^® for PPV detects ALL known strains of the virus including PPV-C, PPV-M, PPV-D, PPV-EA, PPV-Rec, PPV-W, PPV-T, PPV-CR, PPV-An. Click to know more about AmplifyRP®.